{"id":610,"date":"2024-04-25T22:15:17","date_gmt":"2024-04-25T14:15:17","guid":{"rendered":"https:\/\/linguopeng.top\/?p=610"},"modified":"2024-04-28T09:20:41","modified_gmt":"2024-04-28T01:20:41","slug":"%e5%af%b9%e6%b5%8b%e5%ba%8f%e9%a1%ba%e5%ba%8f%e9%87%8d%e6%96%b0%e6%8e%92%e5%88%97%e9%a1%ba%e5%ba%8f","status":"publish","type":"post","link":"https:\/\/linguopeng.top\/?p=610","title":{"rendered":"\u5bf9\u6d4b\u5e8f\u6570\u636e\u91cd\u65b0\u6392\u5217\u987a\u5e8f"},"content":{"rendered":"\n<pre class=\"wp-block-code\"><code>gunzip *\nfor shname in `ls *R1.fastq`;\n   do samplename=`echo \"$shname\" | awk -F_ '{print $1}'`;\n   less $shname|awk '{ if(NR%4==1) gsub(\/@\/,\"@'$samplename'_\"(NR+3)\/4\" \");\nprint $1}'>$samplename.R1.fq ;\ndone\n\nfor shname in `ls *R2.fastq`;\n do samplename=`echo \"$shname\" | awk -F_ '{print $1}'`;\n less $shname|awk '{ if(NR%4==1) gsub(\/@\/,\"@'$samplename'_\"(NR+3)\/4\" \");\n print $1}'>$samplename.R2.fq ;\ndone\n\ngzip *.fq\nls *.R1.fq.gz | awk -F. '{print \"mv \"$0\" \"$1\"_R1.fastq.gz\"}' |bash\nls *.R2.fq.gz | awk -F. '{print \"mv \"$0\" \"$1\"_R2.fastq.gz\"}' |bash\n\nrm *.fastq\n\n#\u57fa\u56e0\u7ec4\nInstrument: Illumina HiSeq 1000\nStrategy: WGS\nSource: GENOMIC\nSelection: RANDOM\nLayout: PAIRED\n#16s\nInstrument: Illumina MiSeq\nStrategy: AMPLICON\nSource: METAGENOMIC\nSelection: PCR\nLayout: PAIRED\n#\u5b8f\u57fa\u56e0\u7ec4\nInstrument: Illumina HiSeq 4000\nStrategy: WGS\nSource: METAGENOMIC\nSelection: cDNA\nLayout: PAIRED\n# Further information and requests for resources and reagents should be directed to Guopeng Lin (7200112079@stu.jiangnan.edu.cn).<\/code><\/pre>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-610","post","type-post","status-publish","format-standard","hentry","category-uncategorized"],"_links":{"self":[{"href":"https:\/\/linguopeng.top\/index.php?rest_route=\/wp\/v2\/posts\/610","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/linguopeng.top\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/linguopeng.top\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/linguopeng.top\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/linguopeng.top\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=610"}],"version-history":[{"count":7,"href":"https:\/\/linguopeng.top\/index.php?rest_route=\/wp\/v2\/posts\/610\/revisions"}],"predecessor-version":[{"id":623,"href":"https:\/\/linguopeng.top\/index.php?rest_route=\/wp\/v2\/posts\/610\/revisions\/623"}],"wp:attachment":[{"href":"https:\/\/linguopeng.top\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=610"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/linguopeng.top\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=610"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/linguopeng.top\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=610"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}